Dear Helen,
No, neither Queen Mary I of England nor Queen Isabella of Spain are official saints of the Church.
That said, there are many, many saints currently in Heaven who were never officially canonized as saints by the Church. When the Church canonizes a person a saint, She holds that person up as an example others should follow but this does not mean there aren't others who have led saintly lives as well.
I can't elaborate further on these people because I do not know the biographical history behind each one. My colleagues may be able to add more.
Here are some informational web pages on the people you asked about:
I hope this helps,
Mike
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